Epigenome editing : methods and protocols / edited by Albert Jeltsch, Marianne G. Rots.
This detailed book explores the concepts and applications of epigenome editing, as presented by leading scientists in the field. Beginning with some general and topical reviews, the collection continues by covering the design of DNA-binding devices, optimization of the effector domains, readout of e...
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| Online Access: |
Full Text (via Springer) |
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| Other Authors: | , |
| Format: | Electronic eBook |
| Language: | English |
| Published: |
New York, NY :
Humana Press : Springer,
[2018]
|
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 1767. |
| Subjects: |
MARC
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| 245 | 0 | 0 | |a Epigenome editing : |b methods and protocols / |c edited by Albert Jeltsch, Marianne G. Rots. |
| 264 | 1 | |a New York, NY : |b Humana Press : |b Springer, |c [2018] | |
| 264 | 4 | |c ©2018 | |
| 300 | |a 1 online resource (xiv, 482 pages) : |b illustrations (some color) | ||
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| 490 | 1 | |a Methods in molecular biology, |x 1940-6029 ; |v volume 1767 | |
| 504 | |a Includes bibliographical references and index. | ||
| 505 | 0 | 0 | |t Editing the epigenome : overview, open questions, and directions of future development / |r Marianne G. Rots and Albert Jeltsch -- |t Zinc fingers, TALEs, and CRISPR systems : a comparison of tools for epigenome editing / |r Charlene Babra Waryah, Colette Moses, Mahira Arooj, and Pilar Blancafort -- |t Designing epigenome editors : considerations of biochemical and locus specificities / |r Dilara Sen and Albert J. Keung -- |t Generation of TALE-based designer epigenome modifiers / |r Sandra Nitsch and Claudio Mussolino -- |t Neuroepigenetic editing / |r Peter J. Hamilton, Carissa J. Lim, Eric J. Nestler, and Elizabeth A. Heller -- |t Allele-specific epigenome editing / |r Pavel Bashtrykov and Albert Jeltsch -- |t Key to delivery : the (Epi- )genome editing vector toolbox / |r Sabrina Just and Hildegard Büning -- |t CRISPR/dCas9 switch systems for temporal transcriptional control / |r Rutger A.F. Gjaltema and Edda G. Schulz -- |t Delivery of designer epigenome modifiers into primary human T cells / |r Tafadzwa Mlambo, Marianna Romito, Tatjana I. Cornu, and Claudio Mussolino -- |t Viral expression of epigenome editing tools in rodent brain using stereotaxic surgery techniques / |r Peter J. Hamilton, Carissa J. Lim, Eric J. Nestler, and Elizabeth A. Heller -- |t Stable expression of epigenome editors via viral delivery and genomic integration / |r Carolin Kroll and Philipp Rathert -- |t Purified protein delivery to activate an epigenetically silenced allele in mouse brain / |r Benjamin Pyles, Barbara J. Bailus, Henriette O'Geen, and David J. Segal -- |t Non-viral methodology for efficient co-transfection / |r Jessica A. Kretzmann, Cameron W. Evans, Marck Norret, Pilar Blancafort, and K. Swaminathan Iyer -- |t Chromatin immunoprecipitation in human and yeast cells / |r Jessica B. Lee and Albert J. Keung -- |t Chromatin immunoprecipitation and high-throughput sequencing (ChIP-Seq) : tips and tricks regarding the laboratory protocol and initial downstream data analysis / |r Darren K. Patten, Giacomo Corleone, and Luca Magnani -- |t Generation of whole genome bisulfite sequencing libraries for comprehensive DNA methylome analysis / |r Dulce B. Vargas-Landin, Jahnvi Pflüger, and Ryan Lister -- |t Approaches for the analysis and interpretation of whole genome bisulfite sequencing data / |r Tim Stuart, Sam Buckberry, and Ryan Lister -- |t Whole-genome bisulfite sequencing for the analysis of genome-wide DNA methylation and hydroxymethylation patterns at single-nucleotide resolution / |r Magali Kernaleguen, Christian Daviaud, Yimin Shen, Eric Bonnet, Victor Renault, Jean-François Deleuze, Florence Mauger, and Jörg Tost -- |t Locus-specific DNA methylation analysis by targeted deep bisulfite sequencing / |r Elsa Leitão, Jasmin Beygo, Michael Zeschnigk, Ludger Klein-Hitpass, Marcel Bargull, Sven Rahmann, and Bernhard Horsthemke -- |t DNA methylation analysis by bisulfite conversion coupled to double multiplexed amplicon-based next-generation sequencing (NGS) / |r Pavel Bashtrykov and Albert Jeltsch -- |t Cell-to-cell transcription variability as measured by single-molecule RNA FISH to detect epigenetic state switching / |r William Beckman, Ilona M. Vuist, Hermannus Kempe, and Pernette J. Verschure -- |t Establishment of cell lines stably expressing dCas9-fusions to address kinetics of epigenetic editing / |r Désirée Goubert, Mihály Koncz, Antal Kiss, and Marianne G. Rots -- |t Editing of DNA methylation using dCas9-peptide repeat and scFv-TET1 catalytic domain fusions / |r Sumiyo Morita, Takuro Horii, and Izuho Hatada -- |t Chemical inducible dCas9-guided editing of H3K27 acetylation in mammalian Cclls / |r Dan Gao and Fu-Sen Liang -- |t Screening regulatory element function with CRISPR/Cas9-based epigenome editing / |r Tyler S. Klann, Gregory E. Crawford, Timothy E. Reddy, and Charles A. Gersbach. |
| 588 | 0 | |a Online resource; title from PDF title page (SpringerProtocol, viewed March 12, 2018). | |
| 520 | |a This detailed book explores the concepts and applications of epigenome editing, as presented by leading scientists in the field. Beginning with some general and topical reviews, the collection continues by covering the design of DNA-binding devices, optimization of the effector domains, readout of epigenome marks, and approaches for delivery at the cellular and organismal level. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Epigenome Editing: Methods and Protocols will be of great assistance to people new to the field but also to those already engaged, as epigenetic editing is still a relatively unexplored field with many issues to be resolved. | ||
| 650 | 0 | |a Epigenetics |v Laboratory manuals. | |
| 650 | 0 | |a Genomics |v Laboratory manuals. | |
| 650 | 7 | |a Epigenetics |2 fast | |
| 650 | 7 | |a Genomics |2 fast | |
| 655 | 7 | |a Laboratory manuals |2 fast | |
| 700 | 1 | |a Jeltsch, Albert, |e editor. | |
| 700 | 1 | |a Rots, Marianne G., |e editor. | |
| 776 | 0 | 8 | |i Print version: |z 9781493977734 |
| 830 | 0 | |a Methods in molecular biology (Clifton, N.J.) ; |v v. 1767. | |
| 856 | 4 | 0 | |u https://colorado.idm.oclc.org/login?url=https://link.springer.com/10.1007/978-1-4939-7774-1 |z Full Text (via Springer) |
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| 956 | |a Springer Protocols | ||
| 956 | |b Springer Nature - Complete Protocols | ||
| 998 | |b Added to collection springerlink.protocols | ||
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