Plastids : methods and protocols / edited by Eric Maréchal.
This volume explores plastid evolution, structure, and function in algae, plants and protists. The methods described in this book help scientists visualize, fractionate, purify, and study primary and secondary plastids in plant and algal materials. The chapters in this book also look at various tech...
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Full Text (via Springer) |
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| Format: | Electronic eBook |
| Language: | English |
| Published: |
New York :
Humana Press : Springer,
[2018]
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| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 1829. |
| Subjects: |
Table of Contents:
- Primary endosymbiosis : emergence of the primary chloroplast and the chromatophore, two independent events / Eric Maréchal
- Complex endosymbioses I : from primary to complex plastids, multiple independent events / Zoltán Füssy and Miroslav Oborník
- Complex endosymbioses II : the nonphotosynthetic plastid of apicomplexa parasites (The apicoplast) and its integrated metabolism / Cyrille Y. Botté and Yoshiki Yamaryo-Botté
- Diversity and plasticity of plastids in land plants / Katalin Solymosi, Johanna Lethin, and Henrik Aronsson
- Main functions of plastids / Norbert Rolland, Imen Bouchnak, Lucas Moyet, Daniel Salvi, and Marcel Kuntz
- Plastid transient and stable interactions with other cell compartments / Stefanie J. Mueller-Schuessele and Morgane Michaud
- Imaging plastids in 2D and 3D : confocal and electron microscopy / Serena Flori, Pierre-Henri Jouneau, Benoit Gallet, Leandro F. Estrozi, Christine Moriscot, Guy Schoehn, Giovanni Finazzi, and Denis Falconet
- Purification of chloroplasts and chloroplast subfractions : envelope, thylakoids, and stroma-from spinach, pea, and Arabidopsis thaliana / Maryse A. Block and Catherine Albrieux
- Isolation of inner and outer membranes of the chloroplast envelope from spinach and pea / Maryse A. Block
- Purification of nongreen plastids (proplastids and amyloplasts) from angiosperms, and isolation of their envelope membranes / Claude Alban and Etienne-Pascal Journet
- In vitro protein import into isolated chloroplasts / Maryse A. Block
- Analysis of the MTL supercomplex at contact sites between mitochondria and plastids / Morgane Michaud
- Isolation of plastid fractions from the diatoms Thalassiosira pseudonana and Phaeodactylum tricornutum / Alexander F. Schober, Serena Flori, Giovanni Finazzi, Peter G. Kroth, and Carolina Río Bártulos
- Isolating the Plasmodium falciparum apicoplast using magnetic beads / Cyrille Y. Botté, Geoffrey I. McFadden, and Yoshiki Yamaryo-Botté
- Extraction and quantification of lipids from plant or algae / Valérie Gros, Josselin Lupette, and Juliette Jouhet
- Quantitative assessment of the chloroplast lipidome / Valérie Gros and Juliette Jouhet
- Determination of the DNA/RNA-associated subproteome from chloroplasts and other plastid types / Maha Chieb, Monique Liebers, Fabien Chevalier, Silva Lerbs-Mache, Robert Blanvillain, and Thomas Pfannschmidt
- Mapping plastid transcript population by circular reverse transcription polymerase chain reaction / Florence Courtois and Livia Merendino
- Bioinformatic analysis of chloroplast gene expression and RNA posttranscriptional maturations using RNA sequencing / Bastien Malbert, Guillem Rigaill, Veronique Brunaud, Claire Lurin, and Etienne Delannoy
- Guide to the chloroplast transcriptome analysis using RNA-Seq / Elena J.S. Michel, Amber M. Hotto, Susan R. Strickler, David B. Stern, and Benoît Castandet
- Toolkit for the characterization of the photoprotective capacity of green algae / M. Águila Ruiz-Sola and Dimitris Petroutsos
- Rescue of deletion mutants to isolate plastid transformants in higher plants / Mohammad El Hajj, Mohd Fahdli Bin Hamdan, Elena Martin Avila, and Anil Day
- Genetic analysis of chloroplast biogenesis, and function and mutant collections / Sho Fujii, Hajime Wada, and Koichi Kobayashi
- Generation of mutants of nuclear-encoded plastid proteins using CRISPR/Cas9 in the diatom Phaeodactylum tricornutum / Guillaume Allorent, Erika Guglielmino, Cécile Giustini, and Florence Courtois.