Imaging flow cytometry : methods and protocols / edited by Natasha S. Barteneva, Ivan A. Vorobjev.
This volume explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionised our ability to analyse cells, cellular clusters and populations. Beginning with an introduction to technology, it continues with sections addressing protocols for studies on th...
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Full Text (via Springer) |
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| Format: | Electronic eBook |
| Language: | English |
| Published: |
New York :
Humana Press,
2016.
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| Series: | Springer protocols (Series)
Methods in molecular biology (Clifton, N.J.) ; v. 1389. |
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Table of Contents:
- Part I: General1. Quantitative Functional Morphology by Imaging Flow Cytometry Ivan A. Vorobjev and Natasha S. Barteneva2. Principles of Amnis Imaging Flow Cytometry David A. Basiji3. Ultrafast Microfluidic Cellular Imaging by Optical Time-Stretch Andy K.S. Lau, Terence T.W. Wong, Ho Cheung Shum, Kenneth K.Y. Wong, and Kevin K. Tsia4. Applications of Imaging Flow Cytometry for Microalgae Mark Hildebrand, Aubrey Davis, Raffaela Abbriano, Haley R. Pugsley, Jesse C. Traller, Sarah R. Smith, Roshan P. Shrestha, Orna Cook, Eva Sanchez, Kalpana Manandhar-Shrestha, and Benjamin AlderetePart II: IFC in Detection of Nuclei and Nucleic Acids5. The Analysis of Cell Cycle, Proliferation, and Asymmetric Cell Division by Imaging Flow Cytometry Andrew Filby, William Day, Sukhveer Purewal, and Nuria Martinez-Martin6. Quantitation of Chromosome Damage by Imaging Flow Cytometry Lindsay A. Beaton-Green and Ruth C. Wilkins7. Fluorescent In Situ Hybridization In Suspension by Imaging Flow Cytometry Orla Maguire, Paul K. Wallace, and Hans Minderman8. Analysis of Nucleo-Cytoplasmic Protein Shuttling by Imaging Flow Cytometry Elizaveta Fasler-Kan, Yeldar Baiken, Ivan Vorobjev, and Natasha S. Barteneva9. Using Image-Based Flow Cytometry with a FISH-Based FlowRNA Assay to Simultaneously Detect Intracellular TNF-α Protein and mRNA in Monocytes Following LPS Stimulation Adam S. Venable, Andrea L. Henning, Eric A. Prado, and Brian K. McFarlinPart III: IFC in Immune Response and Drug Screening10. Multiparametric Characterization of Human T-Cell Immune Synapses by InFlow Microscopy Guido H. Wabnitz and Yvonne Samstag11. Studying T Cells N-Glycosylation by Imaging Flow Cytometry Ana M. Dias, Catarina R. Almeida, Celso A. Reis, and Salomé S. Pinho12. Assessment of Granulocyte Subset Activation: New Information from Image-Based Flow Cytometry Brian K. McFarlin, Eric A. Prado, Adam S. Venable, Andrea L. Henning, and Randall R. Williams13. Using Image-Based Flow Cytometry to Assess Monocyte Oxidized LDL Phagocytosis Capacity Andrea L. Henning, Adam S. Venable, Eric A. Prado, and Brian K. McFarlin14. Screening for Drugs Against the Plasmodium falciparum Digestive Vacuole by Imaging Flow Cytometry Yan Quan Lee, Brian E. Hall, and Kevin S.W. TanPart IV: IFC in Studies of Cell Death and Apoptosis15. Accurate Assessment of Cell Death by Imaging Flow Cytometry Aja M. Rieger and Daniel R. Barreda16. Temporal Heterogeneity in Apoptosis Determined by Imaging Flow Cytometry Ivan A. Vorobjev and Natasha S. BartenevaPart V: Imaging Cytometry in Cell Classification and Morphological Analysis17. FlowCAM: Quantification and Classification of Phytoplankton by Imaging Flow Cytometry Nicole J. Poulton18. Detection and Characterization of Rare Circulating Endothelial Cells by Imaging Flow Cytometry Leigh Samsel and J. Philip McCoy, Jr. 19. Imaging Flow Cytometric Analysis of Primary Bone Marrow Megakaryocytes Lisa M. Niswander, James Palis, and Kathleen E. McGrath20. Sickle Cell Imaging Flow Cytometry Assay (SIFCA) Kleber Y. Fertrin, Leigh Samsel, Eduard J. van Beers, Laurel Mendelsohn, Gregory J. Kato, and J. Philip McCoy.