Plant cell expansion : methods and protocols /

Plant cell expansion : methods and protocols / edited by José M. Estevez.

This volume covers broad aspects of cell expansion in three different cell types: root hairs, pollen tubes, and hypocothyl cells. Chapters focus on the cutting-edge methods to study in detail several complex aspects of cell expansion such as secretion, endocytosis and recycling, cellular signaling a...

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Bibliographic Details
Online Access: Full Text (via Springer)
Other Authors: Estevez, José M.
Format: Electronic eBook
Language:English
Published: New York : Humana Press, ©2015.
Series:Methods in molecular biology (Clifton, N.J.) ; v. 1242.
Subjects:
Plant cells and tissues > Laboratory manuals.
Plant physiology.
Plant physiology
Plant cells and tissues
Laboratory manuals
Table of Contents:
  • Penium margaritaceum as a model organism for cell wall analysis of expanding plant cells / Maja G. Rydahl [and others]
  • Using chemical genomics to study cell wall formation and cell growth in Arabidopsis thaliana and Penium margaritaceum / N. Worden [and others]
  • Optimized method for growing in vitro Arabidopsis thaliana pollen tubes / Cecilia Borassi [and others]
  • Imaging of calcium dynamics in pollen tube cytoplasm / María Laura Barberini and Jorge Muschietti
  • Live imaging of root hairs / Silvia M. Velasquez, Jose R. Dinneny, and José M. Estevez
  • Improved ROS measurement in root hair cells / Paola Silvina Denita Juárez, Silvina Mangano, and José M. Estevez
  • Root hair assay to expedite cell death research / Joanna Kacprzyk and Paul F. McCabe
  • Vacuolar staining methods in plant cells / David Scheuring [and others]
  • Live cell imaging of FM4-64, a tool for tracing the endocytic pathways in Arabidopsis root cells / Adeline Rigal, Siamsa M. Doyle, and Stéphanie Robert
  • Salt-stress regulation of root system growth and architecture in Arabidopsis seedlings / Lina Duan, Jose Sebastian, and Jose R. Dinneny
  • Quantification of fluorescent reporters in plant cells / Michael Pound, Andrew P. French, and Darren M. Wells
  • Live cell imaging of the cytoskeleton and cell wall enzymes in plant cells / Arun Sampathkumar and Raymond Wightman
  • Using the split-ubiquitin yeast two-hybrid system to test protein-protein interactions of transmembrane proteins / Logan Bashline and Ying Gu
  • Activation tag screening for cell expansion genes in Arabidopsis thaliana / Chaowen Xiao and Charles T. Anderson
  • BiFC for protein-protein interactions and protein topology : discussing an integrative approach for an old technique / Giovanni Stefano, Luciana Renna, and Federica Brandizzi
  • N-Glycosylation and plant cell growth / Christiane Veit, Ulrike Vavra, and Richard Strasser
  • Peptide separation methodologies for in-depth proteomics / Sajad Majeed Zargar [and others]
  • Structural and mechanical characterization of growing Arabidopsis plant cell walls / Friederike Saxe, Ingo Burgert, and Michaela Eder.

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