Nuclear G-protein coupled receptors : methods and protocols / edited by Bruce G. Allen, Terence E. Hébert.

Nuclear G-Protein Coupled Receptors: Methods and Protocols is a compilation of a number of conceptual and methodological aspects important for the validation and characterization of intacrine signaling systems. To date, the best-characterized intracrine signaling system is that of angiotensin II (An...

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Bibliographic Details
Online Access: Full Text (via Springer)
Other Authors: Allen, Bruce G. (Editor), Hébert, Terence E. (Editor)
Format: Electronic eBook
Language:English
Published: New York, NY : Humana Press, [2015]
Series:Methods in molecular biology (Clifton, N.J.) ; v. 1234.
Subjects:
Table of Contents:
  • Studies of intracellular angiotensin II / Richard N. Re and Julia L. Cook
  • Single-cell microinjection coupled to confocal microscopy to characterize nuclear membrane receptors in freshly isolated cardiomyocytes / Clémence Merlen and Jonathan Ledoux
  • Design and application of light-activated probes for cellular signaling / David Chatenet, Steve Bourgault, and Alain Fournier
  • Using caged ligands to study intracrine endothelin signaling in intact cardiac myocytes / Clémence Merlen, Louis R. Villeneuve, and Bruce G. Allen
  • Quantification of catecholamine uptake in adult cardiac myocytes / Erika F. Dahl, Casey D. Wright, and Timothy D. O'Connell
  • Characterization of the interaction between the prostaglandin D2 DP1 receptor and the intracellular L-Prostaglandin D synthase / Chantal Binda and Jean-Luc Parent
  • Isolation and study of cardiac nuclei from canine myocardium and adult ventricular myocytes / Artavazd Tadevosyan, Bruce G. Allen, and Stanley Nattel
  • High resolution imaging and function of nuclear G protein-coupled receptors (GPCRs) / Vikrant K. Bhosle [and others]
  • Biochemical fractionation of membrane receptors in the nucleus / Ying-Nai Wang ... [et at.]
  • Functional G protein-coupled Receptors on nuclei from brain and primary cultured neurons / Yuh-Jiin I. Jong and Karen L. O'Malley
  • Automated microscopy of cardiac myocyte hypertrophy : a case study on the role of intracellular [alpha]-adrenergic receptors / Karen A. Ryall and Jeffrey J. Saucerman
  • Measuring intranuclear and nuclear envelope [Ca2+] vs. cytosolic [Ca2+] / Senka Ljubojević and Donald M. Bers
  • Assessing GPCR and G protein signaling to the nucleus in live cells using fluorescent biosensors / Julie Bossuyt and Donald M. Bers
  • Tandem affinity purification to identify cytosolic and nuclear G [beta-gamma]-interacting proteins / Rhiannon Campden [and others]
  • Examining the effects of nuclear GPCRs on gene expression using isolated nuclei / George Vaniotis [and others]
  • Trafficking and function of GPCRs in the endosomal compartment / Davide Calebiro [and others].
  • Studies of intracellular angiotensin II / Richard N. Re and Julia L. Cook
  • Single-cell microinjection coupled to confocal microscopy to characterize nuclear membrane receptors in freshly isolated cardiomyocytes / Clémence Merlen and Jonathan Ledoux
  • Design and application of light-activated probes for cellular signaling / David Chatenet, Steve Bourgault, and Alain Fournier
  • Using caged ligands to study intracrine endothelin signaling in intact cardiac myocytes / Clémence Merlen, Louis R. Villeneuve, and Bruce G. Allen
  • Quantification of catecholamine uptake in adult cardiac myocytes / Erika F. Dahl, Casey D. Wright, and Timothy D. O'Connell
  • Characterization of the interaction between the prostaglandin D2 DP1 receptor and the intracellular L-Prostaglandin D synthase / Chantal Binda and Jean-Luc Parent
  • Isolation and study of cardiac nuclei from canine myocardium and adult ventricular myocytes / Artavazd Tadevosyan, Bruce G. Allen, and Stanley Nattel
  • High resolution imaging and function of nuclear G protein-coupled receptors (GPCRs) / Vikrant K. Bhosle [and others]
  • Biochemical fractionation of membrane receptors in the nucleus / Ying-Nai Wang ... [et at.]
  • Functional G protein-coupled Receptors on nuclei from brain and primary cultured neurons / Yuh-Jiin I. Jong and Karen L. O'Malley
  • Automated microscopy of cardiac myocyte hypertrophy : a case study on the role of intracellular α-adrenergic receptors / Karen A. Ryall and Jeffrey J. Saucerman
  • Measuring intranuclear and nuclear envelope [Ca2+] vs. cytosolic [Ca2+] / Senka Ljubojević and Donald M. Bers
  • Assessing GPCR and G protein signaling to the nucleus in live cells using fluorescent biosensors / Julie Bossuyt and Donald M. Bers
  • Tandem affinity purification to identify cytosolic and nuclear Gβγ-interacting proteins / Rhiannon Campden [and others]
  • Examining the effects of nuclear GPCRs on gene expression using isolated nuclei / George Vaniotis [and others]
  • Trafficking and function of GPCRs in the endosomal compartment / Davide Calebiro [and others].