T follicular helper cells : methods and protocols /

T follicular helper cells : methods and protocols / edited by Marion Espéli, Michelle Linterman.

This volume brings together the skills and protocols of numerous laboratories that are at the heart of investigation into the biology of Tfh cells in both mice and humans. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective top...

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Bibliographic Details
Online Access: Full Text (via Springer)
Other Authors: Espéli, Marion (Editor), Linterman, Michelle (Editor)
Format: Electronic eBook
Language:English
Published: New York, NY : Humana Press, 2015.
Series:Methods in molecular biology (Clifton, N.J.) ; v. 1291.
Subjects:
T cells > Laboratory manuals.
T cells
Laboratory manuals
Table of Contents:
  • Identification of Mouse T Follicular Helper Cells by Flow Cytometry
  • Identification of Follicular T Helper Cells in Tissue Sections
  • Tracking Early T Follicular Helper Cell Differentiation In vivo
  • Tracking by Flow Cytometry Antigen-Specific Follicular Helper T-Cells In Wild-Type Animals After Protein Vaccination
  • Retroviral Vector Expression in TCR Transgenic CD4+ T Cells
  • Two-photon Microscopy For Imaging Germinal Centres and T Follicular Helper Cells
  • In vivo Induction of T-follicular Helper Cells by Modulation of Regulatory T Cell Function
  • Assessing T Follicular Helper Cell Function in vivo: Antigen-specific B Cell Response to Hapten and Affinity Maturation
  • The SWHEL System For High-resolution Analysis of in vivo Antigen-specific T-Dependent B Cell Responses
  • Triggering Positive Selection of Germinal Center B cells by Antigen Targeting to DEC-205
  • Detection of Mouse Natural Killer T Follicular Helper (NKTFH) Cells by Flow Cytometry
  • Identification of Foxp3+ T Follicular Regulatory (Tfr) Cells by Flow Cytometry
  • In vitro Assay to Sensitively Measure Tfr Suppressive Capacity and Tfh Stimulation of B Cell Responses
  • Flow Cytometric Detection and Isolation of Human Tonsil or Lymph Node T Follicular Helper Cells
  • Human Tfh and Tfr cells: Identification and Assessment of their Migration Potential
  • Analysis of Human Blood Memory T Follicular Helper Subsets
  • Flow Cytometric Analysis of Circulating Follicular Helper T (Tfh) and Follicular Regulatory T (Tfr) Populations in Human Blood
  • Quantifying Helper Cell Function of Human TFH Cells in vitro.

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