Hemostasis and thrombosis protocols /

Hemostasis and thrombosis protocols / edited by David J. Perry and K. John Pasi.

In Hemostasis and Thrombosis Protocols, leading experimentalists describe in detail their proven, cutting-edge methods for research on hemostasis and thrombosis, as well as their diagnostics. The techniques range from widely used basic assays to methods that are specialized for mutational analysis a...

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Bibliographic Details
Online Access: Full Text (via EBSCO)
Other Authors: Perry, David (David J.), Pasi, K. John
Format: eBook
Language:English
Published: Totowa, N.J. : Humana Press, ©1999.
Series:Methods in molecular medicine ; 31.
Subjects:
Blood coagulation disorders > Molecular aspects > Laboratory manuals.
Blood > Coagulation > Laboratory manuals.
Thrombosis > Laboratory manuals.
Blood coagulation disorders.
Blood > Diseases > Molecular aspects.
Blood > Coagulation.
Thrombosis.
Laboratory manuals.
Table of Contents:
  • PART I. INTRODUCTION
  • 1. Hemostasis: Components and Processes / K. John Pasi.
  • PART II. BASIC TECHNIQUES
  • 2. Isolation of DNA and RNA / David J. Perry
  • 3. Amplification of DNA and RNA by PCR / David J. Perry
  • 4. Direct Sequencing of PCR Products / David J. Perry
  • 5. Solid-Phase Sequencing of Biotinylated PCR Products with Streptavidin-Coated Magnetic Beads / David J. Perry
  • 6. Automated DNA Sequencing / Helen L. Devereux
  • 7. Detection of DNA by Silver Staining / David J. Perry and Flora Peyvandi
  • 8. Promoter Studies in Hemostasis / Peter R. Winship and Jonathan R.K. Spray.
  • PART III. METHODS OF MUTATIONAL ANALYSIS
  • 9. Detection of Mutations and Polymorphisms in Clotting Factors by Denaturing Gradient Gel Electrophoresis / Rainer Schwaab and Winfried Schmidt
  • 10. Screening for Mutations in DNA by Single-Stranded Conformation Polymorphism (SSCP) Analysis / David J. Perry
  • 11. Screening for DNA Heteroduplexes in the Factor VII Gene Using Ethylene Glycol Gel Electrophoresis of Solvent-Treated ³²P-Labeled PCR Products / Peter Baker
  • 12. Detection of Mutations Causing Hemophilia A Using an In Vitro Coupled Transcription and Translation System / Chike Ononye
  • 13. Screening for Mutations in the Human Antithrombin Gene by Hydrolink D-5000 and MDE Gel Electrophoresis / David J. Perry.
  • PART IV. METHODS FOR ANALYZING INHERITED/ACQUIRED DISORDERS OF HEMOSTASIS
  • 14. Detection of Mutations In Hemophilia A Patients by Chemical Cleavage of Mismatch Method / Naushin H. Waseem, Richard Bagnall, Peter M. Green, and Francesco Giannelli
  • 15. Inversion Mutation Analysis in Hemophilia A by Restriction Enzyme Analysis and Southern Blotting / Chike Ononye
  • 16. Hemophilia B Mutational Analysis / Peter M. Green
  • 17. Screening for Candidate Mutations Causing von Willebrand's Disease (vWD) / P. Vince Jenkins
  • 18. Use of Intron 40 VNTR I in vWD Gene Tracking / Mohammed S. Enayat and G.J. Surdhar
  • 19. Multimeric Analysis of von Willebrand Factor (vWF) / Mohammed S. Enayat
  • 20. Identification of Mutations in the Human Factor VII Gene / Peter M. Baker
  • 21. Molecular Analysis in Factor XI Deficiency / Karen M. Johnson and John H. McVey
  • 22. Mutational Analysis in Antithrombin Deficiency / David J. Perry
  • 23. Ectopic Transcript Analysis in Human Antithrombin Deficiency / David J. Perry
  • 24. Mutational Analysis of the Human Protein C Gene / Roger Luddington
  • 25. Analysis of the Protein S Gene in Protein S Deficiency / Núria Sala and Yolanda Espinosa-Parrilla
  • 26. Screening for the G to A Transition at Position 20210 in the 3ʹ-Untranslated Region (UTR) of the Prothrombin Gene / Karen P. Brown
  • 27. Screening for the Factor V Leiden Mutation / Karen P. Brown
  • 28. Multiplex PCR for Detection of the Prothrombin 3ʹ UTR (C20210A) Polymorphism and the Factor V Leiden Mutation / Gill Mellars, P. Vince Jenkins, and David J. Perry
  • 29. Isoelectric Focusing and Immunodetection of Plasma Antithrombin / Martina Daly
  • 30. Characterization of Heparin Binding Variants of Antithrombin by Crossed Immunoelectrophoresis in the Presence of Heparin / Martina Daly
  • 31.The Determination of Amino Acid Sequence Abnormalities in Proteins by HPLC Peptide Analysis / David Williamson.
  • PART V. PLATELET AND MEGAKARYOCYTE ANALYSIS
  • 32. Molecular Biological Identification and Characterization of Inherited Platelet Receptor Disorders / Ramesh B. Basani, Mark Richberg, and Mortimer Poncz
  • 33. In Vitro Expansion of Megakaryocytes from Peripheral Blood Hematopoietic Progenitors / Michael A. Thornton and Mortimer Poncz
  • 34. Molecular Biology Studies with Primary Megakaryocytes / Yaping Shou and Mortimer Poncz.

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