Eicosanoid protocols / edited by Elias A. Lianos.
In Eicosanoid Protocols, Elias A. Lianos and a panel of hands-on experts present cutting-edge methods for the study of eicosanoids, including prostaglandins, thromboxanes, and leukotrienes. The readily reproducible methods described hereconcentrate on studying the regulation of expression and functi...
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
©1999.
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| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 120. |
| Subjects: |
Table of Contents:
- Use of a Retroviral Vector for Expression of a Foreign Gene / Brent W. Miller and Aubrey R. Morrison
- Extraction of Eicosanoids from Biological Fluids, Cells, and Tissues / William S. Powell
- Assessment of Cyclooxygenase RNA Expression by Northern Hybridization / Zhonghong Guan and Aubrey R. Morrison
- Immunohistochemical Analysis of Cyclooxygenase Expression in Human Skin / ShaAvhree Y. Buckman [and others]
- Separation of Hydroxyeicosatetraenoic Acids and Leukotrienes Originating from Kidney Tissue by C-18 Reverse-Phase High-Pressure Liquid Chromatography / Mukut Sharma, Beatrice Hucke, and Elias A. Lianos
- Localization and Distribution of Cyclooxygenase-2 in Brain Tissue by Immunohistochemistry / M. Kerry O'Banion and John A. Olschowka
- Assessment of Cyclooxygenase Protein Expression by Western Blotting / Zhonghong Guan and Aubrey R. Morrison
- Assessment of Arachidonic Acid Distribution into Phospholipids of Inflammatory Cells / Alfred N. Fonteh
- Determination of Phospholipase A2s Expression in Mast Cells by Reverse-Transcriptase Polymerase Chain Reaction / Alfred N. Fonteh, William Reed, and James M. Samet
- Enzyme Immunoassays (EIA) of Eicosanoids / Kingsley Yin and Lloyd J. Forman
- Transcellular Regulation of Eicosanoid Biosynthesis / Karsten Gronert [and others]
- Assessment of Cellular Localization of the Thromboxane A2 Receptor by Immunocytochemistry / Samuel C. Blackman [and others]
- Epoxyeicosatrienoic Acids : Biosynthesis, Regulation, and Actions / John D. Imig
- Assessment of the Expression of Prostaglandin Synthase-2 in Swiss 3T3 Fibroblasts / Srinivasa T. Reddy and Harvey R. Herschman
- Assessment of 5-Lipoxygenase Activity and Cellular Distribution / Robert A. Lepley
- Leukotrienes, Lipoxins, and Hydroxyeicosatetraenoic Acids / Eric G. Spokas, Joshua Rokach, and Patrick Y.-K. Wong
- Whole Blood Assays for Evaluation of Thromboxane Synthase Inhibition / Eric G. Spokas [and others]
- Novel Eicosanoids: Isoprostanes and Related Compounds / L. Jackson Roberts II [and others]