Protein sensors and reactive oxygen species. Part A, Selenoproteins and thioredoxin [electronic resource] / edited by Helmut Sies, Lester Packer.
This volume of Methods in Enzymology is concerned with the rapidly developing field of selenoprotein synthesis and its related molecular genetics. Progressive information on the topics of proteins as redox sensors, selenoproteins, and the thioredoxin system is studied using methods such as bioinform...
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| Other Authors: | , |
| Other title: | Selenoproteins and thioredoxin. |
| Format: | Electronic eBook |
| Language: | English |
| Published: |
San Diego, Calif. :
Academic Press,
©2002.
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| Series: | Methods in enzymology ;
347. |
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Table of Contents:
- Front Cover; Protein Sensors and Reactive Oxygen Species; Copyright Page; Table of Contents; Contributors to Volume 347; Preface; Volume in Series; Section I: Selenoproteins; Chapter 1. Selenoprotein Biosynthesis: Purification and Assay of Components Involved in Selenocysteine Biosynthesis and Insertion in Escherichia coli; Chapter 2. Selenocysteine Insertion Sequence Element Characterization and Selenoprotein Expression; Chapter 3. Transfer RNAs That Insert Selenocysteine; Chapter 4. Purification and Analysis of Selenocysteine Insertion Sequence-Binding Protein 2.
- Chapter 5. Nonsense-Mediated Decay: Assaying for Effects on Selenoprotein mRNAsChapter 6. Novel Selenoproteins Identified from Genomic Sequence Data; Chapter 7. Semisynthesis of Proteins Containing Selenocysteine; Chapter 8. Mammalian Selenoprotein Gene Signature: Identification and Functional Analysis of Selenoprotein Genes Using Bioinformatics Methods; Chapter 9. Estimation of Individual Types of Glutathione Peroxidases; Chapter 10. High-Throughput 96-Well Microplate Assays for Determining Specific Activities of Glutathione Peroxidase and Thioredoxin Reductase; Chapter 11. Selenoprotein P.
- Chapter 12. Iodothyronine DeiodinasesChapter 13. Expression and Regulation of Thioredoxin Reductases and Other Selenoproteins in Bone; Chapter 14. Selenoprotein W; Chapter 15. Genetic and Functional Analysis of Mammalian Sep15 Selenoprotein; Chapter 16. Selenocysteine Lyase from Mouse Liver; Chapter 17. Selenocysteine Methyltransferase; Chapter 18. Phospholipid-Hydroperoxide Glutathione Peroxidase in Sperm; Chapter 19. In Vivo Antioxidant Role of Glutathione Peroxidase: Evidence from Knockout Mice.
- Chapter 20. Recombinant Expression of Mammalian Selenocysteine- Containing Thioredoxin Reductase and Other Selenoproteins in Escherichia coliChapter 21. Mammalian Thioredoxin Reductases as Hydroperoxide Reductases; Chapter 22. Tryparedoxin and Tryparedoxin Peroxidase; Chapter 23. Trypanothione and Tryparedoxin in Ribonucleotide Reduction; Chapter 24. Selenium- and Vitamin E-Dependent Gene Expression in Rats: Analysis of Differentially Expressed mRNAs; Section II: Thioredoxin; Chapter 25. Overview; Chapter 26. Thioredoxin and Glutaredoxin Isoforms; Chapter 27. Mammalian Thioredoxin Reductases.
- Chapter 28. Mitochondrial Thioredoxin Reductase and Thiol StatusChapter 29. Protein Electrophoretic Mobility Shift Assay to Monitor Redox State of Thioredoxin in Cells; Chapter 30. Recycling of Vitamin C by Mammalian Thioredoxin Reductase; Chapter 31. Thioredoxin Cytokine Action; Chapter 32. Identification of Thioredoxin-Linked Proteins by Fluorescence Labeling Combined with Isoelectric Focusing/Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis; Chapter 33. Thioredoxin and Mechanism of Inflammatory Response; Chapter 34. Redox State of Cytoplasmic Thioredoxin.