Introduction to protein mass spectrometry / Pradip Kumar Ghosh.
Unlike many other methods which automatically yield an absolutely unique protein name as output, protein mass spectrometry generally requires a deduction of protein identity from determination of peptide fragmentation products. This book enables readers to both understand, and appreciate, how determ...
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| Format: | eBook |
| Language: | English |
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London, UK :
Academic Press is an imprint of Elsevier,
[2015]
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Table of Contents:
- Title page; Table of Contents; Copyright page; Dedication; Preface; Chapter 1: Introduction; Chapter 2: Sample Preparation and Ionization for Mass Spectrometry; 2.1 Separation of protein mixtures; 2.2 Multidimensional separations; 2.3 Proteolysis; 2.4 Ionization of proteins and peptides; Chapter 3: Instruments; 3.1 Mass analysis; 3.2 Some examples of instruments; Chapter 4: Mass Spectrometry of Peptides and Proteins; 4.1 Proteins; 4.2 Alternative analysis methods; 4.3 Quantitation; 4.4 Peptide fragmentation: Experiments; 4.5 Data mining scheme for identifying peptide structural motifs.
- 4.6 Peptide fragmentation: mechanism4.7 Possible pathways in the competition model; 4.8 Studying post translational modifications; 4.9 Chemical cross-linking/mass spectrometry; 4.10 Manual de novo sequencing of peptides; Chapter 5: Examples from Biological Applications; 5.1 Mapping intact protein isoforms using top-down proteomics; 5.2 Quantitative analysis of intact apolipoproteins in human HDL; 5.3 Rapid sequence analysis of some conotoxins
- combination of de novo, bottom-up methods; 5.4 Mass spectrometry of ribosomes; 5.5 Proteins in Purkinje cell post-synaptic densities.
- 5.6 Neurexin-LRRTM2 interaction effect in synapse formation5.7 Rapid analysis of human plasma proteome
- an IMS-IMS-MS application; 5.8 Topology of two transient virus capsid assembly intermediates; 5.9 Mass spectrometry of intact V-type ATPases reveals bound lipids and the effects of nucleotide binding; 5.10 Mass spectrometric imaging of biological material; Chapter 6: Mass Spectrometry-Based Bioinformatics; 6.1 Peptides to proteins; 6.2 MS/MS fragments to peptides to proteins; 6.3 Data-dependent acquisition; 6.4 Targeted acquisition covering SRM and PRM; 6.5 Data-independent acquisition.