Germline development [electronic resource] : methods and protocols / edited by Wai-Yee Chan, Le Ann Blomberg.
Saved in:
| Online Access: |
Online Access |
|---|---|
| Other Authors: | , |
| Format: | Electronic eBook |
| Language: | English |
| Published: |
New York, NY :
Humana Press,
©2012.
|
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 825. |
| Subjects: |
Table of Contents:
- Isolation of fetal gonads from embryos of timed-pregnant mice for morphological and molecular studies
- Neonatal testicular gonocytes isolation and processing for immunocytochemical analysis
- Isolation of undifferentiated and early differentiating type a spermatogonia from pou5f1-GFP reporter mice
- Isolation of human male germ-line stem cells using enzymatic digestion and magnetic-activated cell sorting
- Isolation and purification of murine male germ cells
- Preparation of enriched mouse syncitia-free pachytene spermatocyte cell suspensions
- Revealing the transcriptome landscape of mouse spermatogonial cells by tiling microarray
- Biochemical characterization of a testis-predominant isoform of n-[alpha] acetyltransferase
- Identification of novel long noncoding RNA transcripts in male germ cells
- Methylation profiling using methylated DNA immunoprecipitation and tiling array hybridization
- Spermatogenesis in cryptorchidism
- In vitro culture of fetal ovaries: A model to study factors regulating early follicular development
- Microspread ovarian cell preparations for the analysis of meiotic prophase progression in oocytes with improved recovery by cytospin centrifugation
- In vitro maturation (IVM) of porcine oocytes
- Experimental approaches to the study of human primordial germ cells
- Investigating the origins of somatic cell populations in the perinatal mouse ovaries using genetic lineage tracing and immunohistochemistry
- DNA methylation analysis of germ cells by using bisulfite-based sequencing methods
- Gene expression in mouse oocytes by RNA-Seq.