High-throughput next generation sequencing [electronic resource] : methods and applications / edited by Young Min Kwon, Steven C. Ricke.

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Bibliographic Details
Online Access: Online Access
Other Authors: Kwon, Young Min, Ricke, Steven C., 1957-
Format: Electronic eBook
Language:English
Published: New York, N.Y. : Humana Press, ©2011.
Series:Springer protocols (Series)
Methods in molecular biology (Clifton, N.J.) ; v. 733.
Subjects:
Table of Contents:
  • Helicos single-molecule sequencing of bacterial genomes / Kathleen E. Steinmann [and others]
  • Whole-genome sequencing of unculturable bacterium using whole-genome amplification / Yuichi Hongoh and Atsushi Toyoda
  • RNA sequencing and quantitation using the helicos genetic analysis system / Tal Raz [and others]
  • Transcriptome profiling using single-molecule direct RNA sequencing / Fatih Ozsolak and Patrice M. Milos
  • Discovery of bacterial sRNAs by high-throughput sequencing / Jane M. Liu and Andrew Camilli
  • Identification of virus encoding microRNAs using 454 FLX sequencing platform / Byung-Whi Kong
  • Ribosomal RNA depletion for massively parallel bacterial RNA-sequencing applications / Zhoutao Chen and Xiaoping Duan
  • Integrating high-throughput pyrosequencing and quantitative real-time PCR to analyze complex microbial communities / Husen Zhang [and others]
  • Tag-encoded FLX amplicon pyrosequencing for the elucidation of microbial and functional gene diversity in any environment / Yan Sun, Randall D. Wolcott, and Scot E. Dowd
  • Pyrosequencing of chaperonin-60 (cpn60) amplicons as a means of determining microbial community composition / John Schellenberg [and others]
  • Prescreening of microbial populations for the assessment of sequencing potential / Irene B. Hanning and Steven C. Ricke
  • Metagenomics / Jack A. Gilbert [and others]
  • Metagenomic analysis of intestinal microbiomes in chickens / Taejoong Kim and Egbert Mundt
  • Gene expression profiling : metatranscriptomics / Jack A. Gilbert and Margaret Hughes
  • High-throughput insertion tracking by deep sequencing for the analysis of bacterial pathogens / Sandy M.S. Wong [and others]
  • Determining DNA methylation profiles using sequencing / Suhua Feng [and others]
  • Preparation of next-generation sequencing libraries using Nextera technology : simultaneous DNA fragmentation and adaptor tagging by in vitro transposition / Nicholas Caruccio
  • Amplification-free library preparation for paired-end illumina sequencing / Iwanka Kozarewa and Daniel J. Turner
  • Target-enrichment through amplification of hairpin-ligated universal targets for next-generation sequencing analysis / Pallavi Singh, Rajesh Nayak, and Young Min Kwon
  • 96-plex molecular barcoding for the illumina genome analyzer / Iwanka Kozarewa and Daniel J. Turner.