Microsomal lipid peroxidation as a mechanism of cellular damage. [Dissertation] [electronic resource]
The NADPH/iron-dependent peroxidation of lipids in rat liver microsomes was found to be dependent on the presence of free ferrous ion and maintains iron in the reduced Fe/sup 2 +/ state. Chelation of iron by EDTA inhibited peroxidation. Addition of iron, after preincubation of microsomes in the abse...
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Online Access |
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| Corporate Author: | |
| Format: | Government Document Electronic eBook |
| Language: | English |
| Published: |
Normal, Ala. : Oak Ridge, Tenn. :
Alabama A & M University ; distributed by the Office of Scientific and Technical Information, U.S. Department of Energy,
1979.
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| Subjects: |
MARC
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| 088 | |a ur-3490-1765 | ||
| 245 | 0 | 0 | |a Microsomal lipid peroxidation as a mechanism of cellular damage. [Dissertation] |h [electronic resource] |
| 260 | |a Normal, Ala. : |b Alabama A & M University ; |a Oak Ridge, Tenn. : |b distributed by the Office of Scientific and Technical Information, U.S. Department of Energy, |c 1979. | ||
| 300 | |a Pages: 142 : |b digital, PDF file. | ||
| 336 | |a text |b txt |2 rdacontent. | ||
| 337 | |a computer |b c |2 rdamedia. | ||
| 338 | |a online resource |b cr |2 rdacarrier. | ||
| 500 | |a Published through SciTech Connect. | ||
| 500 | |a 01/01/1979. | ||
| 500 | |a "ur-3490-1765" | ||
| 500 | |a Kornbrust, D.J. | ||
| 520 | 3 | |a The NADPH/iron-dependent peroxidation of lipids in rat liver microsomes was found to be dependent on the presence of free ferrous ion and maintains iron in the reduced Fe/sup 2 +/ state. Chelation of iron by EDTA inhibited peroxidation. Addition of iron, after preincubation of microsomes in the absence of iron, did not enhance the rate of peroxidation suggesting that iron acts by initiating peroxidative decomposition of membrane lipids rather than by catalyzing the breakdown of pre-formed hydroperoxides. Liposomes also underwent peroxidation in the presence of ferrous iron at a rate comparable to intact microsomes and was stimulated by ascorbate. Carbon tetrachloride initiated lipid peroxidation in the absence of free metal ions. Rates of in vitro lipid peroxidation of microsomes and homogenates were found to vary widely between different tissues and species. The effects of paraquat on lipid peroxidation was also studied. (DC) | |
| 536 | |b EY-76-C-02-3490. | ||
| 650 | 7 | |a Peroxidases. |2 local. | |
| 650 | 7 | |a Coenzymes. |2 local. | |
| 650 | 7 | |a Organic Compounds. |2 local. | |
| 650 | 7 | |a Mammals. |2 local. | |
| 650 | 7 | |a Microsomes. |2 local. | |
| 650 | 7 | |a Heterocyclic Acids. |2 local. | |
| 650 | 7 | |a Organs. |2 local. | |
| 650 | 7 | |a Organoids. |2 local. | |
| 650 | 7 | |a Lipids. |2 local. | |
| 650 | 7 | |a Organic Acids. |2 local. | |
| 650 | 7 | |a Halogenated Aliphatic Hydrocarbons. |2 local. | |
| 650 | 7 | |a Liver. |2 local. | |
| 650 | 7 | |a Carbon Tetrachloride. |2 local. | |
| 650 | 7 | |a Peroxides. |2 local. | |
| 650 | 7 | |a Organic Halogen Compounds. |2 local. | |
| 650 | 7 | |a Chelating Agents. |2 local. | |
| 650 | 7 | |a Oxygen Compounds. |2 local. | |
| 650 | 7 | |a Vitamins. |2 local. | |
| 650 | 7 | |a Response Modifying Factors. |2 local. | |
| 650 | 7 | |a Enzymes. |2 local. | |
| 650 | 7 | |a Edta. |2 local. | |
| 650 | 7 | |a Biological Variability. |2 local. | |
| 650 | 7 | |a Elements. |2 local. | |
| 650 | 7 | |a Animals. |2 local. | |
| 650 | 7 | |a Rodents. |2 local. | |
| 650 | 7 | |a Iron. |2 local. | |
| 650 | 7 | |a Biological Pathways. |2 local. | |
| 650 | 7 | |a Amino Acids. |2 local. | |
| 650 | 7 | |a Esters. |2 local. | |
| 650 | 7 | |a Cell Constituents. |2 local. | |
| 650 | 7 | |a Organic Nitrogen Compounds. |2 local. | |
| 650 | 7 | |a Metals. |2 local. | |
| 650 | 7 | |a Heterocyclic Compounds. |2 local. | |
| 650 | 7 | |a Carboxylic Acids. |2 local. | |
| 650 | 7 | |a Chlorinated Aliphatic Hydrocarbons. |2 local. | |
| 650 | 7 | |a Nucleotides. |2 local. | |
| 650 | 7 | |a Oxidoreductases. |2 local. | |
| 650 | 7 | |a Rats. |2 local. | |
| 650 | 7 | |a Nadp. |2 local. | |
| 650 | 7 | |a Glands. |2 local. | |
| 650 | 7 | |a Digestive System. |2 local. | |
| 650 | 7 | |a Body. |2 local. | |
| 650 | 7 | |a Vertebrates. |2 local. | |
| 650 | 7 | |a Porphyrins. |2 local. | |
| 650 | 7 | |a Organic Chlorine Compounds. |2 local. | |
| 650 | 7 | |a Ascorbic Acid. |2 local. | |
| 650 | 7 | |a Transition Elements. |2 local. | |
| 650 | 7 | |a Metabolism. |2 local. | |
| 650 | 7 | |a Basic Biological Sciences. |2 edbsc. | |
| 710 | 2 | |a Alabama A & M University. |4 res. | |
| 710 | 1 | |a United States. |b Department of Energy. |b Office of Scientific and Technical Information. |4 dst. | |
| 856 | 4 | 0 | |u http://www.osti.gov/servlets/purl/5825958/ |z Online Access |
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