Flux analysis of central metabolic pathways in the Fe(III)-reducing organism Geobacter metallireducens via 13C isotopiclabeling [electronic resource]

We analyzed the carbon fluxes in the central metabolism ofGeobacter metallireducens strain GS-15 using 13C isotopomer modeling.Acetate labeled in the 1st or 2nd position was the sole carbon source,and Fe-NTA was the sole terminal electron acceptor. The measured labeledacetate uptake rate was 21 mmol...

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Bibliographic Details
Online Access: Online Access
Corporate Author: Lawrence Berkeley National Laboratory (Researcher)
Format: Government Document Electronic eBook
Language:English
Published: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Biological and Environmental Research ; distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2007.
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MARC

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245 0 0 |a Flux analysis of central metabolic pathways in the Fe(III)-reducing organism Geobacter metallireducens via 13C isotopiclabeling  |h [electronic resource] 
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500 |a Applied Environmental Microtiology 73 12 ISSN 0099-2240 FT. 
500 |a Journal Publication Date: June 2007. 
500 |a Hazen, Terry C.; Keasling, Jay D.; Tang, Yinjie J.; Chakraborty, Romy; Chu,Jeannie; Martin, Hector Garcia. 
520 3 |a We analyzed the carbon fluxes in the central metabolism ofGeobacter metallireducens strain GS-15 using 13C isotopomer modeling.Acetate labeled in the 1st or 2nd position was the sole carbon source,and Fe-NTA was the sole terminal electron acceptor. The measured labeledacetate uptake rate was 21 mmol/gdw/h in the exponential growth phase.The resulting isotope labeling pattern of amino acids allowed an accuratedetermination of the in vivo global metabolic reaction rates (fluxes)through the central metabolic pathways using a computational isotopomermodel. The model indicated that over 90 percent of the acetate wascompletely oxidized to CO2 via a complete tricarboxylic acid (TCA) cyclewhile reducing iron. Pyruvate carboxylase and phosphoenolpyruvatecarboxykinase were present under these conditions, but enzymes in theglyoxylate shunt and malic enzyme were absent. Gluconeogenesis and thepentose phosphate pathway were mainly employed for biosynthesis andaccounted for less than 3 percent of total carbon consumption. The modelalso indicated surprisingly high reversibility in the reaction betweenoxoglutarate and succinate. This step operates close to the thermodynamicequilibrium possibly because succinate is synthesized via a transferasereaction, and its product, acetyl-CoA, inhibits the conversion ofoxoglutarate to succinate. These findings enable a better understandingof the relationship between genome annotation and extant metabolicpathways in G. metallireducens. 
520 0 |a Labeled Acetate Electron Acceptor Minimal Medium Tca Cycleacetyl-coa Transferase. 
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650 7 |a Phosphates.  |2 local. 
650 7 |a Binding Energy.  |2 local. 
650 7 |a Biological Pathways.  |2 local. 
650 7 |a Carboxylase.  |2 local. 
650 7 |a Carbon.  |2 local. 
650 7 |a In Vivo.  |2 local. 
650 7 |a Acetates.  |2 local. 
650 7 |a Amino Acids.  |2 local. 
650 7 |a Bypasses.  |2 local. 
650 7 |a Valence.  |2 local. 
650 7 |a Transferases.  |2 local. 
650 7 |a Strains.  |2 local. 
650 7 |a Enzymes.  |2 local. 
650 7 |a Pentoses.  |2 local. 
650 7 |a Biosynthesis.  |2 local. 
650 7 |a Carbon Sources.  |2 local. 
650 7 |a Reaction Kinetics.  |2 local. 
650 7 |a Metabolism.  |2 local. 
650 7 |a Thermodynamics.  |2 local. 
650 7 |a Electrons.  |2 local. 
650 7 |a Iron.  |2 local. 
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