RHIZOSPHERE MICROBIOLOGY OF CHLORINATED ETHENE CONTAMINATED SOILS [electronic resource] : EFFECTS ON PHOSPHOLIPID FATTY ACID CONTENT.
Microbial degradation of chlorinated ethenes (CE) in rhizosphere soils was investigated at seepline areas impacted by CE plumes. Successful bioremediation of CE in rhizosphere soils is dependent on microbial activity, soil types, plant species, and groundwater CE concentrations. Seepline soils were...
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| Format: | Government Document Electronic eBook |
| Language: | English |
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Washington, D.C : Oak Ridge, Tenn. :
United States. Dept. of Energy ; distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy,
2005.
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| Subjects: |
MARC
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| 245 | 0 | 0 | |a RHIZOSPHERE MICROBIOLOGY OF CHLORINATED ETHENE CONTAMINATED SOILS |h [electronic resource] : |b EFFECTS ON PHOSPHOLIPID FATTY ACID CONTENT. |
| 260 | |a Washington, D.C : |b United States. Dept. of Energy ; |a Oak Ridge, Tenn. : |b distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, |c 2005. | ||
| 336 | |a text |b txt |2 rdacontent. | ||
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| 500 | |a 05/26/2005. | ||
| 500 | |a "wsrc-ms-2004-00922" | ||
| 500 | |a Berry, C. J.; Brigmon, R. L.; Franck, M. M.; McKinsey, P. C.; Stanhopc, A. | ||
| 520 | 3 | |a Microbial degradation of chlorinated ethenes (CE) in rhizosphere soils was investigated at seepline areas impacted by CE plumes. Successful bioremediation of CE in rhizosphere soils is dependent on microbial activity, soil types, plant species, and groundwater CE concentrations. Seepline soils were exposed to trichloroethylene (TCE) and perchloroethylene (PCE) in the 10-50 ppb range. Greenhouse soils were exposed to 2-10 ppm TCE. Plants at the seepline were poplar and pine while the greenhouse contained sweet gum, willow, pine, and poplar. Phospholipid fatty acid (PLFA) analyses were performed to assess the microbial activity in rhizosphere soils. Biomass content was lowest in the nonvegetated control soil and highest in the Sweet Gum soil. Bacterial rhizhosphere densities, as measured by PLFA, were similar in different vegetated soils while fungi biomass was highly variable. The PLFA soil profiles showed diverse microbial communities primarily composed of Gram-negative bacteria. Adaptation of the microbial community to CE was determined by the ratio of ω7t/ω7c fatty acids. Ratios (16:1ω7v16:1ω7c and 18:lω7t/18:1ω7c) greater than 0.1 were demonstrated in soils exposed to higher CE concentrations (10-50 ppm), indicating an adaptation to CE resulting in decreased membrane permeability. Ratios of cyclopropyl fatty acids showed that the vegetated control soil sample contained the fastest microbial turnover rate and least amount of environmental stress. PLFA results provide evidence that sulfate reducing bacteria (SRB) are active in these soils. Microcosm studies with these soils showed CE dechlorinating activity was occurring. This study demonstrates microbial adaptation to environmental contamination and supports the application of natural soil rhizosphere activity as a remedial strategy. | |
| 536 | |b DE-AC09-96SR18500. | ||
| 650 | 7 | |a Bacteria. |2 local. | |
| 650 | 7 | |a Poplars. |2 local. | |
| 650 | 7 | |a Membranes. |2 local. | |
| 650 | 7 | |a Carboxylic Acids. |2 local. | |
| 650 | 7 | |a Soils. |2 local. | |
| 650 | 7 | |a Contamination. |2 local. | |
| 650 | 7 | |a Phospholipids. |2 local. | |
| 650 | 7 | |a Fungi. |2 local. | |
| 650 | 7 | |a Greenhouse Gases. |2 local. | |
| 650 | 7 | |a Plumes. |2 local. | |
| 650 | 7 | |a Biomass. |2 local. | |
| 650 | 7 | |a Permeability. |2 local. | |
| 650 | 7 | |a Bioremediation. |2 local. | |
| 650 | 7 | |a Sweet Gums. |2 local. | |
| 650 | 7 | |a Sulfate-reducing Bacteria. |2 local. | |
| 650 | 7 | |a Microcosms. |2 local. | |
| 650 | 7 | |a Biomass Fuels. |2 edbsc. | |
| 710 | 1 | |a United States. |b Department of Energy. |b Savannah River Site. |4 res. | |
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| 710 | 1 | |a United States. |b Department of Energy. |b Office of Scientific and Technical Information. |4 dst. | |
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