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In vivo metabolism of I-123 labeled semisynthetic low density lipoproteins [electronic resource]

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Bibliographic Details
Online Access: Online Access
Corporate Authors: University of Chicago (Researcher), United States. Department of Energy. Chicago Operations Office (Researcher)
Format: Government Document Electronic eBook
Language:English
Published: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Defense ; distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 1990.
Subjects:
Labelled Compounds.
Beta Decay Radioisotopes.
In Vivo.
Organic Compounds.
Biological Half-life.
Document Types.
Mammals.
Drugs.
Lipids.
Organic Acids.
Chemical Preparation.
Labelling.
Electron Capture Radioisotopes.
Primates.
Isotopes.
Tissue Distribution.
Tyrosine.
Proteins.
Animals.
Radiopharmaceuticals.
Iodine Isotopes.
Intermediate Mass Nuclei.
Amino Acids.
Progress Report.
Odd-even Nuclei.
Iodine 123.
Tracer Techniques.
Man.
Lipoproteins.
Carboxylic Acids.
Synthesis.
Hydroxy Acids.
Isotope Applications.
Vertebrates.
Distribution.
Rabbits.
Nuclei.
Metabolism.
Catabolism.
Radioisotopes.
Hours Living Radioisotopes.
Radiology And Nuclear Medicine.
Description
Abstract:We previously observed that small model beta-strand peptides (MBPs) selectively bind to human low density lipoprotein (hLDL) in vitro, and that some MBPs can be labeled with I-123-tyramine cellobiose (I-123-TyC). We hypothesized that metabolism of semisynthetic hLDL should mimic that of covalently labeled native hLDL, and planned to evaluate the biodistribution in rabbits of semisynthetic hLDL; to determine effects of prior oxidation and acetylation of the adsorbing hLDL on binding of MBPs and upon biodistribution of semisynthetic particles; and to begin biodistribution studies with semisynthetic hLDL in human subjects, with the eventual goal of application to experimental and clinical nuclear imaging studies. We have synthesized a radioiodotyrosine-containing MBP, designated betay, as a more suitable adsorbant to hLDL thanradioiodine-TyC-MBP, and optimized conditions for preparing radioiodine-betaY:hLDL. In rabbits both betaY and betaY:hLDL complexes were cleared from the bloodstream much more rapidly than radioiodine-TyC-hLDL or In-111-hLDL, and betaY in either form showed a biodistribution pattern different from that of directly radiolabeled hLDL. Even though radioiodine-betaY can be quickly and easily produced, we conclude that neither betaY alone nor semisynthetic betaY:hLDL particles are likely to prove useful as tracers of hLDL metabolism in vivo.
Item Description:Published through the Information Bridge: DOE Scientific and Technical Information.
12/01/1990.
"doe/er/60725-1"
"DE92040343"
Harper, P.V.
Physical Description:Pages: (10 p) : digital, PDF file.

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