In vivo metabolism of I-123 labeled semisynthetic low density lipoproteins. Final technical report, September 28, 1988--September 27, 1990 [electronic resource]
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| Online Access: |
Online Access |
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| Corporate Authors: | , |
| Format: | Government Document Electronic eBook |
| Language: | English |
| Published: |
Washington, D.C. : Oak Ridge, Tenn. :
United States. Dept. of Energy ; distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy,
1990.
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| Subjects: |
| Abstract: | We previously observed that small model beta-strand peptides (MBPs) selectively bind to human low density lipoprotein (hLDL) in vitro, and that some MBPs can be labeled with I-123-tyramine cellobiose (I-123-TyC). We hypothesized that metabolism of semisynthetic hLDL should mimic that of covalently labeled native hLDL, and planned to evaluate the biodistribution in rabbits of semisynthetic hLDL; to determine effects of prior oxidation and acetylation of the adsorbing hLDL on binding of MBPs and upon biodistribution of semisynthetic particles; and to begin biodistribution studies with semisynthetic hLDL in human subjects, with the eventual goal of application to experimental and clinical nuclear imaging studies. We have synthesized a radioiodotyrosine-containing MBP, designated betay, as a more suitable adsorbant to hLDL thanradioiodine-TyC-MBP, and optimized conditions for preparing radioiodine-betaY:hLDL. In rabbits both betaY and betaY:hLDL complexes were cleared from the bloodstream much more rapidly than radioiodine-TyC-hLDL or In-111-hLDL, and betaY in either form showed a biodistribution pattern different from that of directly radiolabeled hLDL. Even though radioiodine-betaY can be quickly and easily produced, we conclude that neither betaY alone nor semisynthetic betaY:hLDL particles are likely to prove useful as tracers of hLDL metabolism in vivo. |
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| Item Description: | Published through the Information Bridge: DOE Scientific and Technical Information. 12/01/1990. "doe/er/60725--1" "DE92040343" Harper, P.V. |
| Physical Description: | 10 p. : digital, PDF file. |
| Type of Report and Period Covered Note: | Final; |