Transmethylation reactions during methanogenesis from acetate in Methanosarcina barkeri. [Annual report], August 1, 1991--April 1, 1993 [electronic resource]
Methanosarcina possesses an abundance corrinoid, which acts as enzyme prosthetic group and functions in methyl transfer reactions. When this work was initiated only two corrinoid proteins had been described from Methanosarcina: methyltransferase I, which catalyses the first step m methanogenesis fro...
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Online Access |
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Corporate Authors: | , |
Format: | Government Document Electronic eBook |
Language: | English |
Published: |
Washington, D.C. : Oak Ridge, Tenn. :
United States. Dept. of Energy ; distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy,
1993.
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Subjects: |
MARC
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245 | 0 | 0 | |a Transmethylation reactions during methanogenesis from acetate in Methanosarcina barkeri. [Annual report], August 1, 1991--April 1, 1993 |h [electronic resource] |
260 | |a Washington, D.C. : |b United States. Dept. of Energy ; |a Oak Ridge, Tenn. : |b distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, |c 1993. | ||
300 | |a 36 p. : |b digital, PDF file. | ||
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500 | |a 07/01/1993. | ||
500 | |a "doe/er/20042--1" | ||
500 | |a "DE93017203" | ||
500 | |a Krzycki, J.A. | ||
513 | |a Annual; |b 01/01/1991 - 12/31/1993. | ||
520 | 3 | |a Methanosarcina possesses an abundance corrinoid, which acts as enzyme prosthetic group and functions in methyl transfer reactions. When this work was initiated only two corrinoid proteins had been described from Methanosarcina: methyltransferase I, which catalyses the first step m methanogenesis from methanol; and the corrinoid/iron sulfur protein, which is the primary methyl accepting group in the cleavage of acetyl-CoA during methanogenesis from acetate. Using our technique of in vitro methylation of corrinoid proteins by radioactive methanogenic substrates we have found several novel corrinoid proteins are present in this organism which can function as methytransferases, which are methylated by growth substrate, and are apparently regulated by growth substrate. | |
536 | |b FG02-91ER20042. | ||
650 | 7 | |a Biological Pathways. |2 local. | |
650 | 7 | |a Autoradiography. |2 local. | |
650 | 7 | |a Carbon 14 Compounds. |2 local. | |
650 | 7 | |a Progress Report. |2 local. | |
650 | 7 | |a Electrophoresis. |2 local. | |
650 | 7 | |a Tracer Techniques. |2 local. | |
650 | 7 | |a Fractionation. |2 local. | |
650 | 7 | |a Methanogenic Bacteria. |2 local. | |
650 | 7 | |a Gel Permeation Chromatography. |2 local. | |
650 | 7 | |a Methyl Transferases. |2 local. | |
650 | 7 | |a Biomass Fuels. |2 edbsc. | |
710 | 2 | |a Ohio State University. |b Department of Microbiology. |4 res. | |
710 | 1 | |a United States. |b Department of Energy. |4 spn. | |
710 | 1 | |a United States. |b Department of Energy. |b Chicago Operations Office. |4 res. | |
710 | 1 | |a United States. |b Department of Energy. |b Office of Scientific and Technical Information. |4 dst. | |
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