Craniofacial development : methods and protocols /

Craniofacial development : methods and protocols / edited by Sebastian Dworkin.

This volume explores scientific methodologies currently employed to integrate observational developmental biology, tissue explant and cell-based approaches and genetic/molecular technologies to develop a holistic understanding of craniofacial development. Chapters guide readers through the use of di...

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Bibliographic Details
Other Authors: Dworkin, Sebastian (Editor)
Format: Book
Language:English
Published: New York, NY : Humana Press, [2022]
Series:Methods in molecular biology (Clifton, N.J.) ; v. 2403.
Springer protocols (Series)
Subjects:
Skull > Growth.
Facial bones > Growth.
Developmental biology.
Cytology.
Table of Contents:
  • 1. Using Caenorhabditis elegans as a model for mechanistic insights of craniofacial development
  • Wholemount In-situ Hybridisation (WISH) in Zebrafish Embryos to Analyse Craniofacial Developmen
  • Elucidationof Gene Expression Patterns in The Craniofacial Tissues Of Mouse Embryos By Whole-MountIn Situ Hybridization
  • Visualizationof the Cartilage and Bone Elements in The Craniofacial Structures by Alcian blue and Alizarin Red Staining
  • Methodology for Free-Floating Organ Culture of Mid-Gestation Maxillary Primordial Tissue
  • Methods of Palate Culture in Later Palatogenesis: Elevation, Horizontal Outgrowth, and Fusion
  • Single cell RNA-seq: Cell Isolation and Data Analysis
  • Generating Zebrafish RNA-less Mutant Alleles by Deleting Gene Promoters with CRISPR/Cas9
  • Craniofacial Phenomics: Three-Dimensional Assessment of the Size and Shape of Cranial and Dentofacial Structures
  • Micro-CT-based Bone Microarchitecture Analysis of the Murine Skull
  • Characterization of mammalian in vivo enhancers using mouse transgenesis and CRISPR genome editing
  • Histological Techniques for Sectioning Bones of the Vertebrate Craniofacial Skeleton
  • Engineering Epithelial-Mesenchymal Microtissues to Study Cell-Cell Interactions in Development
  • Ex Vivo Culture of Human Cranial Suture Cells
  • Scaffolds for Use in Craniofacial Bone Regeneration
  • Mandible Explant Assay for The Analysis of Meckel's Cartilage Development
  • High Resolution Histology for Craniofacial Studies On Zebrafish and Other Teleost Models
  • Live Imaging the Dynamics Of Mammalian Neural Crest Cell Migration
  • Salivary Gland Development In Culture
  • Antenatal Ultrasound Imaging for Analysis of Human Craniosynostosis.

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