Fluorescence microscopy : super-resolution and other novel techniques /

Fluorescence microscopy : super-resolution and other novel techniques / edited by Anda Cornea and P. Michael Conn.

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Fluorescence Microscopy: Super-Resolution and other Novel Techniques delivers a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science. With contributions selected for clarity, utility, and reproducibility, the work provides practi...

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Bibliographic Details
Online Access: Full Text (via ProQuest)
Other Authors: Cornea, Anda (Editor), Conn, P. Michael (Editor)
Format: eBook
Language:English
Published: London : Academic/Elsevier Press, 2014.
Subjects:
Fluorescence microscopy.
Table of Contents:
  • Front Cover; Fluorescence Microscopy: Super-Resolution and Other Novel Techniques; Copyright; Contents; Preface; List of Contributors; Chapter 1
  • Evanescent Excitation and Emission; INTRODUCTION; EVANESCENCE IN GENERAL; EVANESCENCE IN EXCITATION: TIR; EVANESCENCE IN EMISSION: THE FLUOROPHORE NEAR FIELD; EVANESCENCE FROM A POINT SOURCE: NSOM; Acknowledgments; References; Chapter 2
  • Adaptive Optics for Fluorescence Microscopy; INTRODUCTION; ABERRATIONS IN MICROSCOPY; PRINCIPLES OF ADAPTIVE OPTICS; ADAPTIVE ABERRATION CORRECTION IN FLUORESCENCE MICROSCOPES; WAVEFRONT SENSING.
  • SCHEMES FOR MODAL SENSORLESS ADAPTIVE OPTICSCONCLUSION; References; Chapter 3
  • Rapid Measurements of Orientation and Rotation in Ex Vivo Muscle Made Possible by Studying Small Number of Cross-Bridges; INTRODUCTION; KINETICS; DISTRIBUTION OF CROSS-BRIDGE ORIENTATIONS; CONCLUSIONS; Acknowledgments; References; Chapter 4
  • High Spatiotemporal Bioimaging Techniques to Study the Plasma Membrane Nanoscale Organization; INTRODUCTION; SPATIAL RESOLUTION; TEMPORAL RESOLUTION; CONCLUSIONS AND OUTLOOK; References; Chapter 5
  • High-Resolution 3D Imaging of Intact Transparent Organs by 3DISCO.
  • INTRODUCTIONCURRENT TECHNOLOGIES FOR IMAGING THE INTACT BRAIN; 3DISCO; Acknowledgments; References; Chapter 6
  • Using RNA Mimics of GFP to Image RNA Dynamics in Mammalian Cells; INTRODUCTION; STUDYING TRINUCLEOTIDE-REPEAT-CONTAINING RNA; OPTIMIZING IMAGING OF CGG REPEAT-CONTAINING RNA; TIME-LAPSE IMAGING OF CGG-REPEAT-CONTAINING RNAS; MONITORING CGG REPEAT FOCI DURING CELL DIVISION; EXAMINING THE STABILITY OF CGG60 RNA; IMAGING THE EFFECTS OF SMALL-MOLECULE DRUGS ON CGG FOCI; IMAGING OTHER RNAS USING SPINACH2; TROUBLESHOOTING IMAGING WITH SPINACH2-TAGGED CONSTRUCTS; IMAGING RARE RNAS.
  • FUTURE DIRECTIONSReferences; Chapter 7
  • Surface Enhanced Raman Scattering (SERS) Image Cytometry for High-Content Screening; HIGH-CONTENT SCREENING: STATUS AND NEEDS; LABELS AND PROBES FOR HCS: FLUORESCENCE AND SERS; SPECTRAL IMAGING FOR FLUORESCENCE AND SERS; SERS IMAGING IN CELL-BASED ASSAYS; STATUS AND PROSPECTS; Acknowledgments; References; APPENDIX; Chapter 8
  • Light Sheet Fluorescence Microscopy Applications for Multicellular Systems; INTRODUCTION; LSFM IMPLEMENTATIONS: A UNIVERSAL CONCEPT WITH MANY ACRONYMS; LSFM AT THE MACROSCOPIC SCALE; 3D CANCER IMAGING MODEL: MCTS.
  • CALCIUM IMAGING, FAST VOLUMETRIC CALCIUM IMAGINGCONCLUSIONS; Acknowledgments; References; Chapter 9
  • Correlative Light Electron Microscopy as a Navigating Tool for Cryo-Electron Tomography Analysis; INTRODUCTION; ELECTRON MICROSCOPY IN CELLULAR AND STRUCTURAL BIOLOGY; SAMPLE PREPARATION FOR ELECTRON MICROSCOPY; CRYO-ELECTRON TOMOGRAPHY; CORRELATIVE LIGHT AND ELECTRON MICROSCOPY (CLEM); CORRELATED LIGHT AND ELECTRON MICROSCOPY DIRECT OBSERVATION IN CRYOGENIC TEMPERATURES; CLEM PROSPECTS; Acknowledgments; References.
  • Chapter 10
  • Cellular and Molecular Applications of Super-resolution Microscopy.

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