Alf1p, a CLIP-170 Domain-containing Protein, Is Functionally and Physically Associated with ?-Tubulin [electronic resource]
Tubulin is a heterodimer of ?- and ?-tubulin polypeptides. Assembly of the tubulin heterodimer in vitro requires the CCT chaperonin complex, and a set of five proteins referred to as the tubulin cofactors (Tian, F., Y. Huang, H. Rommelaere, J. Vandekerckhove, C. Ampe, and N.J. Cowan. 1996. Cell. 86:...
Saved in:
| Online Access: |
Full Text (via OSTI) |
|---|---|
| Corporate Author: | |
| Format: | Government Document Electronic eBook |
| Language: | English |
| Published: |
Washington, D.C. : Oak Ridge, Tenn. :
United States. Department of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Department of Energy,
1999.
|
| Subjects: |
MARC
| LEADER | 00000nam a22000003u 4500 | ||
|---|---|---|---|
| 001 | b11823656 | ||
| 003 | CoU | ||
| 005 | 20220922040000.0 | ||
| 006 | m o d f | ||
| 007 | cr ||||||||||| | ||
| 008 | 221019e19990111||| o| f0|||||eng|d | ||
| 035 | |a (TOE)ost1625111 | ||
| 035 | |a (TOE)1625111 | ||
| 040 | |a TOE |c TOE | ||
| 049 | |a GDWR | ||
| 072 | 1 | |a 59 |2 edbsc | |
| 086 | 0 | |a E 1.99:1625111 | |
| 086 | 0 | |a E 1.99:1625111 | |
| 245 | 0 | 0 | |a Alf1p, a CLIP-170 Domain-containing Protein, Is Functionally and Physically Associated with ?-Tubulin |h [electronic resource] |
| 260 | |a Washington, D.C. : |b United States. Department of Energy. Office of Science ; |a Oak Ridge, Tenn. : |b Distributed by the Office of Scientific and Technical Information, U.S. Department of Energy, |c 1999. | ||
| 300 | |a Size: p. 113-124 : |b digital, PDF file. | ||
| 336 | |a text |b txt |2 rdacontent. | ||
| 337 | |a computer |b c |2 rdamedia. | ||
| 338 | |a online resource |b cr |2 rdacarrier. | ||
| 500 | |a Published through Scitech Connect. | ||
| 500 | |a 01/11/1999. | ||
| 500 | |a "Journal ID: ISSN 0021-9525." | ||
| 500 | |a Feierbach, Becket ; Nogales, Eva ; Downing, Kenneth H. ; Stearns, Tim ; | ||
| 520 | 3 | |a Tubulin is a heterodimer of ?- and ?-tubulin polypeptides. Assembly of the tubulin heterodimer in vitro requires the CCT chaperonin complex, and a set of five proteins referred to as the tubulin cofactors (Tian, F., Y. Huang, H. Rommelaere, J. Vandekerckhove, C. Ampe, and N.J. Cowan. 1996. Cell. 86:287?296; Tian, G., S.A. Lewis, B. Feierbach, T. Stearns, H. Rommelaere, C. Ampe, and N.J. Cowan. 1997. J. Cell Biol. 138:821?832). We report the characterization of Alf1p, the yeast ortholog of mammalian cofactor B. Alf1p interacts with ?-tubulin in both two-hybrid and immunoprecipitation assays. Alf1p and cofactor B contain a single CLIP-170 domain, which is found in several microtubule-associated proteins. Mutation of the CLIP-170 domain in Alf1p disrupts the interaction with ?-tubulin. Mutations in ?-tubulin that disrupt the interaction with Alf1p map to a domain on the cytoplasmic face of ?-tubulin; this domain is distinct from the region of interaction between ?-tubulin and ?-tubulin. Alf1p-green fluorescent protein (GFP) is able to associate with microtubules in vivo, and this localization is abolished either by mutation of the CLIP-170 domain in Alf1p, or by mutation of the Alf1p-binding domain in ?-tubulin. Analysis of double mutants constructed between null alleles of ALF1 and PAC2, which encodes the other yeast ?-tubulin cofactor, suggests that Alf1p and Pac2p act in the same pathway leading to functional ?-tubulin. The phenotype of overexpression of ALF1 suggests that Alf1p can act to sequester ?-tubulin from interaction with ?-tubulin, raising the possibility that it plays a regulatory role in the formation of the tubulin heterodimer. | |
| 536 | |b SC0012704. | ||
| 650 | 7 | |a 59 basic biological sciences |2 local. | |
| 650 | 7 | |a Cell biology |2 local. | |
| 650 | 7 | |a Tubulin |2 local. | |
| 650 | 7 | |a Microtubule |2 local. | |
| 650 | 7 | |a Saccharomyces cerevisiae |2 local. | |
| 650 | 7 | |a Chaperonin |2 local. | |
| 650 | 7 | |a Clip170 |2 local. | |
| 650 | 7 | |a Basic biological sciences |2 local. | |
| 650 | 7 | |a Clip-170 |2 local. | |
| 710 | 2 | |a Brookhaven National Laboratory. |4 res. | |
| 710 | 1 | |a United States. |b Department of Energy. |b Office of Science. |4 spn. | |
| 710 | 1 | |a United States. |b Department of Energy. |b Office of Scientific and Technical Information |4 dst. | |
| 856 | 4 | 0 | |u https://www.osti.gov/servlets/purl/1625111 |z Full Text (via OSTI) |
| 907 | |a .b118236568 |b 02-28-23 |c 05-05-21 | ||
| 998 | |a web |b 12-08-22 |c f |d m |e p |f eng |g |h 0 |i 2 | ||
| 956 | |a Information bridge | ||
| 999 | f | f | |i 9e1c240b-95c5-5214-ba8b-9b5edafd45bf |s 1662a083-cf3b-5915-b762-7815a2f5e886 |
| 952 | f | f | |p Can circulate |a University of Colorado Boulder |b Online |c Online |d Online |e E 1.99:1625111 |h Superintendent of Documents classification |i web |n 1 |