Chromatin and chromosomal protein research / edited by Gary Stein and Janet Stein, Lewis J. Kleinsmith.
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| Online Access: |
Full Text (via Internet Archive) |
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| Other Authors: | , , |
| Format: | Government Document eBook |
| Language: | English |
| Published: |
New York :
Academic Press,
1977-78.
|
| Series: | Methods in cell biology ;
v. 16-19. |
| Subjects: |
Table of Contents:
- V. 16. Methods for isolation of nuclei and nucleoli
- Nonaqueous isolation of nuclei from cultured cells
- Isolation of nuclei using hexylene glycol
- Isolation of nuclei and preparation of chromatin from plant tissues
- Methods for the isolation of nuclei from ciliated protozoans
- The isolation of nuclei from fungi
- Methods for nuclear transplantation in amphibia
- Methods for studying nucleocytoplasmic exchange of nonhistone proteins in embryos
- Methods for studying nucleocytoplasmic exchange of macromolecules
- Histone nomenclature
- The isolaltion and purification of histones
- Fractionation of histones on molecular sieve matrices
- Chromatographic fractionation of histones
- Cytochemical quantitation of histones
- The isolation and purification of the high mobility group (HMG) nonhistone chromosomal proteins
- Identification of contractile proteins in nuclear protein fractions
- Methods for selective extraction of chromosomal nonhistone proteins
- Low-molecular-weight basic proteins in spermatids
- Isolation and characterization of nonhistone phosphoproteins
- Fractionation of nonhistone chromosomal proteins utilizing hydroxyapatite chromatography
- Isolation of nuclear proteins associated with the nuclear pore complex and the nuclear peripheral lamina of rat liver
- Posttranscriptional modifications of nonhistone proteins of salivary gland cells of sciara coprophila
- The mitotic apparatus: methods for isolation
- Isolation of the mitotic apparatus
- Fractionation of nonhistone chromosomal proteins utilizing isoelectric focusing techniques
- The rapid isolation, high-resolution electrophoretic characterization, and purification of nuclear proteins
- Two-dimensional plyacrylamide gel electrophoretic fractionation
- Methods for fractionation of chromatin into transcriptionally active and inactive segments
- Chromatin fractionation by chromatography on ECTHAM-cellulose
- Fractionation of chromatin in a two-phase aqueous polymer system
- Biochemical approaches to chromatin assembly
- Nuclease digestion of RNA in chromatin. v. 17. Procedures for minimizing protease activity during isolation of nuclei, chromatin, and the histones
- Isolation of nuclei from animal cells in culture
- Preparation of chromatin from animal tissues and cultured cells
- Methods for isolation of nuclei from cultural mammalian cells: conditions for preferential retention of selected histones
- Methods for isolation of nuclei from spermatozoa
- Manual enucleation of xenopus oocytes
- Macro and micro methods for isolation and injection of cell components from polytene tissues
- Isolation of nuclei and chromatin from Phycomyces blakesleeanus
- Mammaliam metaphase chromosomes
- Analytical techniques for isolated metaphase chromosome fractions
- Methods and mechanisms of chromosome banding
- Cytological analysis of drosophila polytene chromosomes
- Isolation and purification of nucleoli and nucleolar chromatin from mammalian cells
- Nucleolar proteins
- Electrophoretic fractionation of histones utilizing starch gels and sodium dodecyl sulfate-urea gels
- Resolution of histones by polyacrylamide gel electrophoresis in presence of nonionic detergents
- Polyacrylamide gel electrophoretic fractionation of histones
- Affinity chromatography of DNA-binding proteins on DNA covalently attached to solid supports
- DNA-binding proteins
- Fractionation of nonhistone proteins by histone-affinity chromatography
- Fractionation of nonhistone chromosomal proteins
- Fractionation of chromosomal nonhistone proteins using chromatin-cellulose resins: purification of steroid hormone acceptor proteins
- Methods for assessing the binding of steroid hormones in nuclei
- Methods for assessing the binding of steroid hormones in nuclei and chromatin
- Proteins of nuclear ribonucleoprotein subcomplexes
- Isolation and characterization of ribonucleoprotein particles containing heterogeneous nuclear RNA
- Chromosomal association of an Epstein-Barr virus-associated nuclear antigen. v. 18. Fractionation of chromatin into template-active template-inactive portions
- Fractionation of chromatin by buoyant density-gradient sedimentation in metrizamide
- dissection of the eukaryotic chromosome with deoxyribonucleases
- Isolation of amplified nucleoli from xenopus oocytes
- Visualization of chromatin v-bodies
- Isolation and characterization of chromatin subunits
- Serological analyses of histones
- Immunochemical analysis of nonhistone proteins
- Radioimmunoassay of nonhistone proteins
- Immunofluorescent techniques in the analysis of chromosomal proteins
- Peptide mapping and amino acid sequencing of histones
- Determination of the primary structures of histones
- Preparation of chromatin and chromosomes for electron microscopy
- Flow-cytometric analysis of chromatin
- The laser microbeam as a probe for chromatin structure and function
- Utilization of neutron scattering for analysis of chromatin and nucleoprotein structure
- Circular dichroism analysis of chromatin and DNA-nuclear protein complexes
- Intercalating agents as probes of chromatin structure
- Thermal denaturation analysis of chromatin and DNA-nuclear protein complexes
- Assaying histone interactins by sedimentation equilibrium and other methods
- The study of histone-histone associations by chemical cross-linking. v. 19. Isolation and assay of eukaryotic DNA-dependent RNA polymerases
- Isolation and characterization of DNA polymerases from eukaryotic cells
- Methods for assessment of DNase activity
- Assessment of acetylation in nonhistone chromosomal proteins
- Purification and characterization of protein methylase I (S-Adenosylmethionine: protein-arginine methyltransferase; EC 2.1.1.23) from calf brain
- The methylation and demethylation of protein lysine residues
- Purification and assay of protein methylase II (S-adenosylmethionine: protein-carboxyl methyltransferase; EC 2.1.1.24)
- Assessment of methylation in nonhistone chromosomal proteins
- Methods for the assessment of histone mnethylation
- Purification and assay of nuclear protein kinases
- Phosphorylation of nonhistone chromosomal proteins by nuclear phosphokinases covalently linked to agarose
- Methods for the assessment of nonhistone phosphorylation (acid-stable, alkali-labile linkages)
- Methods for the assessment of site-specific histone phosphorylation
- Isolation of histone kinases
- Chromosomal protein phosphorylation on basic amino acids
- Assay of nonhistone phosphatase activity
- ADP-ribosylation of nuclear proteins
- Chromatin-bound proteases and their inhibitors
- Histone hydrolase
- Translation of HeLa cell histone messenger RNAs in cell-free protein synthesizing systems from rabbit reticulocytes, HeLa cells, and wheat germ
- Isolation of histone messenger RNA and its translation in vitro
- In vitro synthesis of single-stranded DNA complementary to histone messenger RNAs
- Immunological methods for the isolation of histone H5 mRNA from chicken reticulocytes
- Enrichment and purification of sea urchin histone genes
- Visualization of genes by electron microscopy
- Techniques of in vitro RNA synthesis with isolate nucleoli
- Transcription of RNA in isolated nuclei
- Transcription of chromatin with heterologous and homologous RNA polymerases
- Transcription of chromatin and prokaryotic and eukaryotic polymerases
- Transcription of chromatin from cells: transformed by SV40 virus
- Chromatin reconstitution
- Methods for dissociation, fractionation, and selective reconstitution of chromatin
- The assay of globin gene transcription in reconstituted chromatin.