Chromatin and chromosomal protein research / edited by Gary Stein and Janet Stein, Lewis J. Kleinsmith.

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Bibliographic Details
Online Access: Full Text (via Internet Archive)
Other Authors: Stein, Gary S., Stein, Janet L., Kleinsmith, Lewis J.
Format: Government Document eBook
Language:English
Published: New York : Academic Press, 1977-78.
Series:Methods in cell biology ; v. 16-19.
Subjects:

MARC

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245 0 0 |a Chromatin and chromosomal protein research /  |c edited by Gary Stein and Janet Stein, Lewis J. Kleinsmith. 
260 |a New York :  |b Academic Press,  |c 1977-78. 
300 |a 1 online resource (4 volumes :  |b illustrations) 
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490 1 |a Methods in cell biology ;  |v v. 16-19. 
504 |a Includes bibliographical references and indexes. 
505 0 |a V. 16. Methods for isolation of nuclei and nucleoli -- Nonaqueous isolation of nuclei from cultured cells -- Isolation of nuclei using hexylene glycol -- Isolation of nuclei and preparation of chromatin from plant tissues -- Methods for the isolation of nuclei from ciliated protozoans -- The isolation of nuclei from fungi -- Methods for nuclear transplantation in amphibia -- Methods for studying nucleocytoplasmic exchange of nonhistone proteins in embryos -- Methods for studying nucleocytoplasmic exchange of macromolecules -- Histone nomenclature -- The isolaltion and purification of histones -- Fractionation of histones on molecular sieve matrices -- Chromatographic fractionation of histones -- Cytochemical quantitation of histones -- The isolation and purification of the high mobility group (HMG) nonhistone chromosomal proteins -- Identification of contractile proteins in nuclear protein fractions -- Methods for selective extraction of chromosomal nonhistone proteins -- Low-molecular-weight basic proteins in spermatids -- Isolation and characterization of nonhistone phosphoproteins -- Fractionation of nonhistone chromosomal proteins utilizing hydroxyapatite chromatography -- Isolation of nuclear proteins associated with the nuclear pore complex and the nuclear peripheral lamina of rat liver -- Posttranscriptional modifications of nonhistone proteins of salivary gland cells of sciara coprophila -- The mitotic apparatus: methods for isolation -- Isolation of the mitotic apparatus -- Fractionation of nonhistone chromosomal proteins utilizing isoelectric focusing techniques -- The rapid isolation, high-resolution electrophoretic characterization, and purification of nuclear proteins -- Two-dimensional plyacrylamide gel electrophoretic fractionation -- Methods for fractionation of chromatin into transcriptionally active and inactive segments -- Chromatin fractionation by chromatography on ECTHAM-cellulose -- Fractionation of chromatin in a two-phase aqueous polymer system -- Biochemical approaches to chromatin assembly -- Nuclease digestion of RNA in chromatin. v. 17. Procedures for minimizing protease activity during isolation of nuclei, chromatin, and the histones -- Isolation of nuclei from animal cells in culture -- Preparation of chromatin from animal tissues and cultured cells -- Methods for isolation of nuclei from cultural mammalian cells: conditions for preferential retention of selected histones -- Methods for isolation of nuclei from spermatozoa -- Manual enucleation of xenopus oocytes -- Macro and micro methods for isolation and injection of cell components from polytene tissues -- Isolation of nuclei and chromatin from Phycomyces blakesleeanus -- Mammaliam metaphase chromosomes -- Analytical techniques for isolated metaphase chromosome fractions -- Methods and mechanisms of chromosome banding -- Cytological analysis of drosophila polytene chromosomes -- Isolation and purification of nucleoli and nucleolar chromatin from mammalian cells -- Nucleolar proteins -- Electrophoretic fractionation of histones utilizing starch gels and sodium dodecyl sulfate-urea gels -- Resolution of histones by polyacrylamide gel electrophoresis in presence of nonionic detergents -- Polyacrylamide gel electrophoretic fractionation of histones -- Affinity chromatography of DNA-binding proteins on DNA covalently attached to solid supports -- DNA-binding proteins -- Fractionation of nonhistone proteins by histone-affinity chromatography -- Fractionation of nonhistone chromosomal proteins -- Fractionation of chromosomal nonhistone proteins using chromatin-cellulose resins: purification of steroid hormone acceptor proteins -- Methods for assessing the binding of steroid hormones in nuclei -- Methods for assessing the binding of steroid hormones in nuclei and chromatin -- Proteins of nuclear ribonucleoprotein subcomplexes -- Isolation and characterization of ribonucleoprotein particles containing heterogeneous nuclear RNA -- Chromosomal association of an Epstein-Barr virus-associated nuclear antigen. v. 18. Fractionation of chromatin into template-active template-inactive portions -- Fractionation of chromatin by buoyant density-gradient sedimentation in metrizamide -- dissection of the eukaryotic chromosome with deoxyribonucleases -- Isolation of amplified nucleoli from xenopus oocytes -- Visualization of chromatin v-bodies -- Isolation and characterization of chromatin subunits -- Serological analyses of histones -- Immunochemical analysis of nonhistone proteins -- Radioimmunoassay of nonhistone proteins -- Immunofluorescent techniques in the analysis of chromosomal proteins -- Peptide mapping and amino acid sequencing of histones -- Determination of the primary structures of histones -- Preparation of chromatin and chromosomes for electron microscopy -- Flow-cytometric analysis of chromatin -- The laser microbeam as a probe for chromatin structure and function -- Utilization of neutron scattering for analysis of chromatin and nucleoprotein structure -- Circular dichroism analysis of chromatin and DNA-nuclear protein complexes -- Intercalating agents as probes of chromatin structure -- Thermal denaturation analysis of chromatin and DNA-nuclear protein complexes -- Assaying histone interactins by sedimentation equilibrium and other methods -- The study of histone-histone associations by chemical cross-linking. v. 19. Isolation and assay of eukaryotic DNA-dependent RNA polymerases -- Isolation and characterization of DNA polymerases from eukaryotic cells -- Methods for assessment of DNase activity -- Assessment of acetylation in nonhistone chromosomal proteins -- Purification and characterization of protein methylase I (S-Adenosylmethionine: protein-arginine methyltransferase; EC 2.1.1.23) from calf brain -- The methylation and demethylation of protein lysine residues -- Purification and assay of protein methylase II (S-adenosylmethionine: protein-carboxyl methyltransferase; EC 2.1.1.24) -- Assessment of methylation in nonhistone chromosomal proteins -- Methods for the assessment of histone mnethylation -- Purification and assay of nuclear protein kinases -- Phosphorylation of nonhistone chromosomal proteins by nuclear phosphokinases covalently linked to agarose -- Methods for the assessment of nonhistone phosphorylation (acid-stable, alkali-labile linkages) -- Methods for the assessment of site-specific histone phosphorylation -- Isolation of histone kinases -- Chromosomal protein phosphorylation on basic amino acids -- Assay of nonhistone phosphatase activity -- ADP-ribosylation of nuclear proteins -- Chromatin-bound proteases and their inhibitors -- Histone hydrolase -- Translation of HeLa cell histone messenger RNAs in cell-free protein synthesizing systems from rabbit reticulocytes, HeLa cells, and wheat germ -- Isolation of histone messenger RNA and its translation in vitro -- In vitro synthesis of single-stranded DNA complementary to histone messenger RNAs -- Immunological methods for the isolation of histone H5 mRNA from chicken reticulocytes -- Enrichment and purification of sea urchin histone genes -- Visualization of genes by electron microscopy -- Techniques of in vitro RNA synthesis with isolate nucleoli -- Transcription of RNA in isolated nuclei -- Transcription of chromatin with heterologous and homologous RNA polymerases -- Transcription of chromatin and prokaryotic and eukaryotic polymerases -- Transcription of chromatin from cells: transformed by SV40 virus -- Chromatin reconstitution -- Methods for dissociation, fractionation, and selective reconstitution of chromatin -- The assay of globin gene transcription in reconstituted chromatin. 
650 0 |a Chromatin. 
650 0 |a Chromosomal proteins. 
650 7 |a Chromatin.  |2 fast  |0 (OCoLC)fst00859922. 
650 7 |a Chromosomal proteins.  |2 fast  |0 (OCoLC)fst00860058. 
700 1 |a Stein, Gary S. 
700 1 |a Stein, Janet L. 
700 1 |a Kleinsmith, Lewis J. 
776 0 8 |i Online version:  |t Chromatin and chromosomal protein research.  |d New York : Academic Press, 1977-78  |w (OCoLC)659398674. 
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