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|a QP551
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| 100 |
1 |
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|a Bonner, Philip.
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| 245 |
1 |
0 |
|a Protein Purification.
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| 250 |
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|a 2nd ed.
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| 260 |
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|a Milton :
|b Taylor & Francis Group,
|c 2018.
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| 300 |
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|a 1 online resource (288 pages)
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| 336 |
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|a text
|b txt
|2 rdacontent.
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| 337 |
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|a computer
|b c
|2 rdamedia.
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| 338 |
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|a online resource
|b cr
|2 rdacarrier.
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| 490 |
1 |
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|a The BASICS (Garland Science) Ser.
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| 505 |
0 |
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|a Cover; Half Title; Title Page; Copyright Page; Table of Contents; Detailed Contents; Preface; Abbreviations; Chapter 1: Water, pH and Buffers; 1.1 Introduction; 1.2 The structure of water; 1.3 Water as a Solvent; 1.4 The Ionisation of Water; 1.5 The pH scale; 1.6 Acids and Bases; 1.7 Buffers; 1.7.1 Points to remember about buffers; 1.8 Summary; Exercise 1.1 Practice calculations using the Henderson-Hasselbalch equation; Recommended Reading; Chapter 2: Protein Structure and Properties; 2.1 Introduction; 2.2 Amino Acids; 2.3 The Peptide Bond; 2.4 Overview of Protein Structure.
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| 505 |
8 |
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|a 2.4.1 Primary structure2.4.2 Secondary structure; 2.4.3 Tertiary structure; 2.4.4 Quaternary structure; 2.5 Motifs and domains (super secondary structure); 2.6 Post-translational Modifications; 2.7 The Characteristics of a Protein Which can be Exploited to Purify a Target Protein; 2.7.1 Surface charge; 2.7.2 Hydrophobic nature; 2.7.3 Solubility; 2.7.4 Biospecificity; 2.7.5 Molecular mass (Mr); 2.7.6 Post-translational modifications; 2.7.7 Engineering proteins to aid purification; 2.8 A range of techniques that can be used in protein purification; 2.8.1 Surface charge; 2.8.2 Hydrophobicity.
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| 505 |
8 |
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|a 2.8.3 Biospecificity2.8.4 Molecular mass; 2.9 Summary; Recommended reading; Chapter 3: Chromatography and the Strategy of Protein Purification; 3.1 Introduction; 3.2 Chromatography; 3.3 The Theories of Chromatography; 3.3.1 The plate theory of chromatography; 3.3.2 The rate theory of chromatography; 3.4 Parameters in Chromatography; 3.5 The Strategy of Protein Purification; 3.5.1 Introduction; 3.5.2 Strategy; 3.5.3 The process of protein purification; 3.6 The Equipment Required for Protein Purification; 3.6.1 Columns; 3.6.2 Tubing; 3.6.3 Pumps; 3.6.4 Fraction collectors; 3.6.5 Detectors.
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| 505 |
8 |
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|a 3.6.6 Gradient makers3.6.7 Different elution procedures; 3.6.7.1 Isocratic elution; 3.6.7.2 Stepped isocratic elution; 3.6.7.3 Gradient elution; 3.6.8 Regeneration and storage of chromatographic resins (media); 3.7 Automated Protein Purification Chromatography Systems; 3.8 Simulated moving bed chromatography (SMBC); 3.9 Protein Purification Chromatographic Runs; 3.9.1 Pre-packed chromatographic columns; 3.9.2 Empty chromatography columns; 3.9.3 Flow rates and elution of the sample; 3.10 Scaling Up the Purification and Some Considerations About Industrial Protein Purification; 3.11 Summary.
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| 505 |
8 |
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|a Protocol for Chapter 3Recommended reading; Chapter 4: The Groundwork; 4.1 Introduction; 4.2 Assay to Identify a Target Protein; 4.2.1 Temperature; 4.2.2 Substrate concentration; 4.2.3 pH; 4.3 Protein Assays; 4.4 The Extraction of Protein from Cells or Tissue; 4.4.1 Introduction; 4.4.2 Reducing agents; 4.4.3 Chelating agents; 4.4.4 Enzyme substrates/Inhibitors/Activators/Cofactors; 4.4.5 Inhibitors of peptidase enzymes; 4.4.6 Phosphatase inhibitors; 4.4.7 Removal of nucleic acids and nucleoproteins; 4.4.8 Removal of lipoproteins; 4.4.9 Additions for the extraction of plant tissue.
|
| 500 |
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|a 4.4.10 Additions for the extraction of membrane proteins.
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| 504 |
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|a Includes bibliographical references and index.
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| 520 |
3 |
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|a This second edition of Protein Purification provides a guide to the major chromatographic techniques, including non-affinity absorption techniques, affinity procedures, non-absorption techniques and methods for monitoring protein purity. The new edition of the book has been organized to encourage incremental learning about the topic, starting with the properties of water, progressing through the characteristics of amino acids and proteins which relate to the purification process. There is an overview of protein strategy and equipment, followed by discussions and examples of each technique and their applications. The basic theory and simple explanations given in Protein Purification make it an ideal handbook for final year undergraduates, and postgraduates, who are conducting research projects. It will also be a useful guide to more experienced researchers who need a good overview of the techniques and products used in protein purification.Key Features* Guide to the major techniques used in protein purification* Includes flowcharts to help the reader slect the best purification strategy* Contains step-by-step protocols that guide the reader through each technique and its use* Includes exercises and solutions.
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| 588 |
0 |
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|a Print version record.
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| 650 |
|
0 |
|a Proteins
|x Purification.
|
| 650 |
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0 |
|a Proteins
|x isolation & purification.
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| 650 |
|
7 |
|a Proteins
|x Purification.
|2 fast
|0 (OCoLC)fst01079750.
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| 776 |
0 |
8 |
|i Print version:
|a Bonner, Philip.
|t Protein Purification.
|d Milton : Taylor & Francis Group, ©2018
|z 9780815344889.
|
| 830 |
|
0 |
|a BASICS (Garland Science) Ser.
|
| 856 |
4 |
0 |
|u http://ebookcentral.proquest.com/lib/ucb/detail.action?docID=5491288
|z Full Text (via ProQuest)
|
| 907 |
|
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|a .b103546364
|b 05-30-23
|c 03-04-19
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| 915 |
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|a M
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| 948 |
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|a May 2024 ebook central cleanup, msw.
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| 956 |
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|a Ebook Central
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| 956 |
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|b Ebook Central Perpetual Titles
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|a University of Colorado Boulder
|b Online
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|d Online
|e QP551
|h Library of Congress classification
|i web
|n 1
|
